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pNIC-ZB-COVID-SARS2 NSP13质粒现货

BACKBONE

  • Vector backbone

    pNIC-ZB

  • Backbone manufacturer

    Addgene Plasmid #26107

  • Backbone size w/o insert (bp)5515

  • Total vector size (bp)7327

  • Vector type

    Bacterial Expression

GROWTH IN BACTERIA

  • Bacterial Resistance(s)

    Kanamycin

  • Growth Temperature

    37°C

  • Growth Strain(s)

    DH5alpha

  • Copy number

    Low Copy

GENE/INSERT

  • Gene/Insert name

    Covid-SARS2 Nsp13

  • Species

    Synthetic; COVID-SARS2

  • Insert Size (bp)

    1809

  • Mutation

    Codon-optimized for E. coli expression

  • GenBank ID

    NC_045512.2

  • PromoterT7 - LacO

  • Tag / Fusion Protein

    • His6-Zb-TEV (N terminal on backbone)

CLONING INFORMATION

RESOURCE INFORMATION

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

    质粒序列如下:taatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataCATATGCACCATCATCATCATCATTCTTCTGGTGTGGATAACAAGTTCAACAAGGAGCGTCGAAGAGCTCGCCGTGAAATTCGCCATCTGCCGAACCTGAACCGCGAACAGCGTCGCGCATTTATTCGCAGCCTGCGCGATGATCCGAGCCAGAGCGCGAACCTGCTGGCGGAAGCGAAGAAGCTGAACGATGCGCAGCCGAAGGGTACCGAGAACCTGTACTTCCAATCCATGGCTGTTGGTGCATGCGTTTTGTGTAATAGTCAAACTTCTTTGCGTTGCGGCGCCTGCATCCGCCGCCCATTTCTGTGCTGTAAATGTTGCTACGATCATGTGATTAGCACGTCGCATAAGCTGGTGCTGTCGGTCAACCCGTATGTTTGTAATGCGCCCGGTTGTGACGTCACGGATGTTACCCAGCTCTATCTGGGTGGTATGTCTTACTACTGCAAGTCACATAAGCCGCCGATTTCATTTCCTCTCTGCGCGAATGGCCAGGTGTTTGGTTTGTATAAAAACACCTGCGTGGGGTCTGATAATGTAACCGATTTTAACGCGATTGCTACGTGTGACTGGACCAACGCCGGCGATTATATCCTTGCCAACACCTGCACTGAACGTCTTAAACTCTTCGCGGCCGAAACCCTGAAAGCAACCGAAGAAACGTTCAAACTGAGCTATGGTATCGCGACAGTACGCGAAGTACTCAGTGACCGGGAGTTGCACCTGTCGTGGGAAGTCGGTAAACCTCGCCCCCCGTTAAACCGCAACTACGTGTTCACCGGCTATCGCGTTACCAAAAACAGCAAGGTGCAAATTGGTGAGTACACCTTTGAGAAAGGCGACTATGGTGATGCCGTAGTATACCGCGGCACCACGACTTACAAACTGAATGTGGGTGATTATTTTGTATTAACCTCACATACCGTAATGCCGTTGAGCGCCCCCACCCTCGTACCGCAGGAACACTATGTACGTATCACGGGTTTATACCCGACGCTGAACATCAGCGATGAGTTTAGCAGTAACGTTGCTAACTACCAGAAAGTGGGTATGCAAAAATACTCGACGTTACAGGGCCCGCCAGGTACTGGAAAGTCTCATTTCGCCATCGGCTTAGCCTTATACTATCCATCAGCTCGCATTGTTTACACGGCCTGCTCTCATGCTGCAGTGGACGCCTTATGCGAGAAGGCGTTAAAATATCTGCCGATTGATAAATGCTCCCGTATTATCCCGGCGCGGGCGCGGGTCGAGTGTTTTGACAAATTTAAAGTGAACAGCACACTGGAACAGTATGTTTTTTGTACGGTGAATGCTCTCCCAGAGACAACTGCCGATATCGTCGTGTTCGACGAAATCAGCATGGCCACTAACTACGATCTGTCGGTCGTTAACGCGCGTCTCCGCGCTAAGCATTATGTTTACATTGGCGATCCGGCCCAGCTGCCGGCTCCACGCACGCTCCTGACTAAAGGTACTCTGGAACCGGAATATTTTAATAGCGTATGCCGTCTGATGAAAACGATTGGACCTGATATGTTCCTGGGAACTTGCCGCCGGTGTCCGGCCGAGATCGTTGATACGGTTTCTGCTCTGGTTTATGACAATAAATTAAAAGCTCACAAAGATAAAAGCGCGCAATGCTTCAAAATGTTTTATAAAGGTGTAATTACCCACGATGTGAGTAGCGCGATTAACCGCCCGCAAATTGGCGTTGTCCGTGAATTCCTGACCCGGAATCCTGCGTGGCGCAAAGCAGTGTTCATTAGCCCATATAATAGTCAAAACGCGGTAGCGTCCAAGATTTTGGGTCTGCCGACACAAACCGTGGACTCCAGCCAGGGCAGTGAGTACGACTATGTGATTTTTACCCAGACCACTGAGACAGCACACTCCTGCAACGTGAACCGCTTCAATGTAGCGATCACCCGTGCGAAGGTAGGTATTTTGTGCATCATGTCCGACCGTGATCTGTATGACAAATTGCAGTTTACCAGCCTGGAAATCCCTCGCCGTAACGTGGCAACCCTTCAATAACAGTAAAGGTGGATACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggattggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaat




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