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pNIC-ZB
Addgene Plasmid #26107
Backbone size w/o insert (bp)5515
Total vector size (bp)7327
Bacterial Expression
Kanamycin
37°C
DH5alpha
Low Copy
质粒序列如下：taatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataCATATGCACCATCATCATCATCATTCTTCTGGTGTGGATAACAAGTTCAACAAGGAGCGTCGAAGAGCTCGCCGTGAAATTCGCCATCTGCCGAACCTGAACCGCGAACAGCGTCGCGCATTTATTCGCAGCCTGCGCGATGATCCGAGCCAGAGCGCGAACCTGCTGGCGGAAGCGAAGAAGCTGAACGATGCGCAGCCGAAGGGTACCGAGAACCTGTACTTCCAATCCATGGCTGTTGGTGCATGCGTTTTGTGTAATAGTCAAACTTCTTTGCGTTGCGGCGCCTGCATCCGCCGCCCATTTCTGTGCTGTAAATGTTGCTACGATCATGTGATTAGCACGTCGCATAAGCTGGTGCTGTCGGTCAACCCGTATGTTTGTAATGCGCCCGGTTGTGACGTCACGGATGTTACCCAGCTCTATCTGGGTGGTATGTCTTACTACTGCAAGTCACATAAGCCGCCGATTTCATTTCCTCTCTGCGCGAATGGCCAGGTGTTTGGTTTGTATAAAAACACCTGCGTGGGGTCTGATAATGTAACCGATTTTAACGCGATTGCTACGTGTGACTGGACCAACGCCGGCGATTATATCCTTGCCAACACCTGCACTGAACGTCTTAAACTCTTCGCGGCCGAAACCCTGAAAGCAACCGAAGAAACGTTCAAACTGAGCTATGGTATCGCGACAGTACGCGAAGTACTCAGTGACCGGGAGTTGCACCTGTCGTGGGAAGTCGGTAAACCTCGCCCCCCGTTAAACCGCAACTACGTGTTCACCGGCTATCGCGTTACCAAAAACAGCAAGGTGCAAATTGGTGAGTACACCTTTGAGAAAGGCGACTATGGTGATGCCGTAGTATACCGCGGCACCACGACTTACAAACTGAATGTGGGTGATTATTTTGTATTAACCTCACATACCGTAATGCCGTTGAGCGCCCCCACCCTCGTACCGCAGGAACACTATGTACGTATCACGGGTTTATACCCGACGCTGAACATCAGCGATGAGTTTAGCAGTAACGTTGCTAACTACCAGAAAGTGGGTATGCAAAAATACTCGACGTTACAGGGCCCGCCAGGTACTGGAAAGTCTCATTTCGCCATCGGCTTAGCCTTATACTATCCATCAGCTCGCATTGTTTACACGGCCTGCTCTCATGCTGCAGTGGACGCCTTATGCGAGAAGGCGTTAAAATATCTGCCGATTGATAAATGCTCCCGTATTATCCCGGCGCGGGCGCGGGTCGAGTGTTTTGACAAATTTAAAGTGAACAGCACACTGGAACAGTATGTTTTTTGTACGGTGAATGCTCTCCCAGAGACAACTGCCGATATCGTCGTGTTCGACGAAATCAGCATGGCCACTAACTACGATCTGTCGGTCGTTAACGCGCGTCTCCGCGCTAAGCATTATGTTTACATTGGCGATCCGGCCCAGCTGCCGGCTCCACGCACGCTCCTGACTAAAGGTACTCTGGAACCGGAATATTTTAATAGCGTATGCCGTCTGATGAAAACGATTGGACCTGATATGTTCCTGGGAACTTGCCGCCGGTGTCCGGCCGAGATCGTTGATACGGTTTCTGCTCTGGTTTATGACAATAAATTAAAAGCTCACAAAGATAAAAGCGCGCAATGCTTCAAAATGTTTTATAAAGGTGTAATTACCCACGATGTGAGTAGCGCGATTAACCGCCCGCAAATTGGCGTTGTCCGTGAATTCCTGACCCGGAATCCTGCGTGGCGCAAAGCAGTGTTCATTAGCCCATATAATAGTCAAAACGCGGTAGCGTCCAAGATTTTGGGTCTGCCGACACAAACCGTGGACTCCAGCCAGGGCAGTGAGTACGACTATGTGATTTTTACCCAGACCACTGAGACAGCACACTCCTGCAACGTGAACCGCTTCAATGTAGCGATCACCCGTGCGAAGGTAGGTATTTTGTGCATCATGTCCGACCGTGATCTGTATGACAAATTGCAGTTTACCAGCCTGGAAATCCCTCGCCGTAACGTGGCAACCCTTCAATAACAGTAAAGGTGGATACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggattggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaat
Covid-SARS2 Nsp13
Synthetic; COVID-SARS2
1809
Codon-optimized for E. coli expression
NC_045512.2
PromoterT7 - LacO
Tag / Fusion Protein
His6-Zb-TEV (N terminal on backbone)
Cloning methodLigation Independent Cloning
5′ sequencing primerT7F
3′ sequencing primerT7R
Not Available to Industry
